PCR Equipment and Supplies
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Applied Biosystems™ SYBR™ Select Master Mix for CFX
A cost-effective solution for real-time PCR applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Form | Liquid |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Vial |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast or Standard |
| Label or Dye | SYBR GreenER |
| Hot Start | Built-In Hot Start |
| Product Line | SYBR |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Applied Biosystems™ MeltDoctor™ HRM Master Mix
Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Form | Liquid |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | HRM |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Genotyping Target | SNPs (Unknown or Numerous), Epigenetic Targets |
| Product Type | HRM Master Mix |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | High Resolution Melting (HRM),SYTO 9 |
| Technique | HRM (High Resolution Melting) Analysis, Mutation Scanning |
| Product Line | MeltDoctor |
Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Type | Universal Master Mix II |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Product Line | TaqMan |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Form | Liquid |
| Gene Symbol | ACTB |
| Amplicon Size | 171 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | NM_001101.2 |
| Target | β-actin |
| Format | Tube |
| Label or Dye | FAM |
| Species | Human |
| Product Line | FAM, TaqMan |
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Activation Time | 5 min. |
| Final Product Type | Mastermix |
| Form | Liquid |
| Reaction Speed | Fast |
| Label or Dye | ROX |
| Hot Start | Built-in Hot Start |
| Concentration | 2X |
| Starting Material | DNA/RNA |
| Compatibility | TaqMan™ |
| For Use With (Application) | Animal Health |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Cloning,Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Thermo Scientific™ Taq Buffers (10X)
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.
| Physical Form | Liquid |
|---|---|
| Quantity | 4 x 1.25 mL |
| Concentration | 10X |
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Taq DNA Polymerase |
| Product Type | One-Step qRT-PCR Kit |
| For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
| Sample Type | RNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
| PCR Method | 1-step RT-qPCR |
| Product Line | Platinum, SuperScript |
| Detection Method | Primer-probe |
Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)
Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RT-PCR Assay Kit
Detect RNA from multiple respiratory viruses simultaneously with the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit, a highly accurate, real-time PCR multiplex assay that allows you to expand your respiratory sample analysis menu while maintaining low operational costs.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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