PCR Equipment and Supplies
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Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Fidelity (vs. Taq) | 1X |
|---|---|
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Starting Material | DNA |
| Exonuclease Activity | 5' - 3' |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ M-MLV Reverse Transcriptase Buffer
M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ T7 Promoter Primer
Thermo Fisher Scientific offers primers for PCR amplification that complement many of the vectors currently available.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ MeltDoctor™ HRM Master Mix
Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Form | Liquid |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | HRM |
| Polymerase | AmpliTaq Gold 360 DNA Polymerase |
| Genotyping Target | SNPs (Unknown or Numerous), Epigenetic Targets |
| Product Type | HRM Master Mix |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | High Resolution Melting (HRM),SYTO 9 |
| Technique | HRM (High Resolution Melting) Analysis, Mutation Scanning |
| Product Line | MeltDoctor |
Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Product Type | Quantitative PCR SuperMix-UDG |
| For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | Platinum |
| Detection Method | Primer-probe |
Thermo Scientific™ dUTP Solution (100 mM)
Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Applied Biosystems™ VetMAX™-Plus qPCR Master Mix
For use with VetMAX™ Reagents and VetMAX™ Controls.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Path-ID™ qPCR Master Mix
Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Purity or Quality Grade | UP (Ultra Pure) |
| Format | Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Form | Liquid |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast or Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Animal Health,Pathogen Detection |
| PCR Method | qPCR |
| Product Line | Ambion, Path-ID |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript II |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Form | Liquid |
| Gene Symbol | GAPDH |
| Amplicon Size | 122 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | NM_002046.3 |
| Target | GAPDH |
| Format | Tube |
| Label or Dye | FAM |
| Species | Human |
| Product Line | FAM, TaqMan |